COMPETITIVE RNA TEMPLATES FOR DETECTION AND QUANTITATION OF GROWTH FACTORS, CYTOKINES, EXTRACELLULAR MATRIX COMPONENTS AND MATRIX METALLOPROTEINASES BY RT-PCR

Detection of low-abundance mRNAs by reverse transcription-polymerase chain reaction (RT-PCR) has become a standard technique to determine gene expression by tissues and cells in culture.The ability to determine relative or absolute copy number of specific mRNAs has been difJicult due to inadequate internal standards to control for sample-to-sample

Objective This study investigated the characteristics and survival rates of patients with intentional severe trauma (self-harm or suicide) who were transported to either a regional trauma center (TC) or a non-TC facility.Methods This retrospective, national, population-based, observational, case-control study included patients who sustained intenti